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Article

Identification and cloning of genes from Porphyromonas gingivalis after mutagenesis with a modified Tn4400 transposon from Bacteroides fragilis.

Infection and immunity - 1 Jan 2000

Chen T, Dong H, Tang Y P, Dallas M M, Malamy M H, Duncan M J

Abstract excerpt

Porphyromonas gingivalis is a gram-negative, black-pigmented, oral anaerobe strongly associated with adult periodontitis. Previous transposon mutagenesis studies with this organism were based on the Bacteroides transposon Tn4351. Characterization of Tn4351-disrupted genes by cloning has not been an efficient way to analyze large numbers of mutants and is further complicated by the high rate of cointegration of...

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Identifiers

PMID: 10603421
DOI: 10.1128/IAI.68.1.420-423.2000
PMCID: PMC97154

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