Identification and cloning of genes from Porphyromonas gingivalis after mutagenesis with a modified Tn4400 transposon from Bacteroides fragilis.

doi:10.1128/IAI.68.1.420-423.2000

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Article

Identification and cloning of genes from Porphyromonas gingivalis after mutagenesis with a modified Tn4400 transposon from Bacteroides fragilis.

Infection and immunity - 1 Jan 2000

Chen T, Dong H, Tang Y P, Dallas M M, Malamy M H, Duncan M J

Abstract excerpt

Porphyromonas gingivalis is a gram-negative, black-pigmented, oral anaerobe strongly associated with adult periodontitis. Previous transposon mutagenesis studies with this organism were based on the Bacteroides transposon Tn4351. Characterization of Tn4351-disrupted genes by cloning has not been an efficient way to analyze large numbers of mutants and is further complicated by the high rate of cointegration of...

Topics

Adult
Bacteroides fragilis
Base Sequence
Cloning, Molecular
DNA Primers
DNA Transposable Elements
DNA, Bacterial
Escherichia coli
Genes, Bacterial
Humans
Mutagenesis, Insertional

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Periodontitis

Phenotype

Pigmentation

Porphyromonas gingivalis

Virulence

Source

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Identifiers

PMID: 10603421
DOI: 10.1128/IAI.68.1.420-423.2000
PMCID: PMC97154

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