Analysis of duplex DNA by triple helix formation: application to detection of a p53 microdeletion.

Article

Analysis of duplex DNA by triple helix formation: application to detection of a p53 microdeletion.

BioTechniques - 1 Jan 1994

Olivas W M, Maher L J

Abstract excerpt

Conventional methods for mutation detection include Southern hybridization, direct sequencing of PCR products and single-strand conformation polymorphism analysis. We present an additional screening method that employs oligonucleotide-directed DNA triple helix formation to detect mutations within...

Topics

Base Sequence
DNA
Gene Deletion
Genes, p53
Molecular Sequence Data
Mutation
Nucleic Acid Conformation
Oligonucleotide Probes

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.