Article
Homogeneous amplification and mutation scanning of the p53 gene using fluorescent melting curves.
Clinical chemistry - 1 Aug 2002
Millward Haleigh, Samowitz Wade, Wittwer Carl T, Bernard Philip S
Abstract excerpt
BACKGROUND: In malignancy, gene mutations frequently occur in tumor suppressor genes such as p53 and are sporadically located. We describe a homogeneous method for amplification and mutation scanning, and apply the method to the p53 gene. METHODS: Using a series of overlapping fluorescein-labeled oligonucleotides complementary to a wild-type p53 sequence, we detected somatic mutations in colorectal cancers by...
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