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Article

Single-step DGGE-based mutation scanning of the p53 gene: application to genetic diagnosis of colorectal cancer.

Human mutation - 1 Jan 1997

Guldberg P, Nedergaard T, Nielsen H J, Olsen A C, Ahrenkiel V, Zeuthen J

Abstract excerpt

We present a simple nonradioactive assay based on polymerase chain reaction (PCR) in combination with denaturing gradient gel electrophoresis (DGGE), which allows comprehensive mutation scanning of the entire coding sequence and splice junctions of the p53 gene in one analytical step. The key features of the present method are (1) all fragments can be amplified using one common PCR protocol; (2) all fragments can...

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Identifiers

PMID: 9101296
DOI: 10.1002/(SICI)1098-1004(1997)9:4<348::AID-HUMU8>3.0.CO;2-1

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