Single-step DGGE-based mutation scanning of the p53 gene: application to genetic diagnosis of colorectal cancer.

doi:10.1002/(SICI)1098-1004(1997)9:4<348::AID-HUMU8>3.0.CO;2-1

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Article

Single-step DGGE-based mutation scanning of the p53 gene: application to genetic diagnosis of colorectal cancer.

Human mutation - 1 Jan 1997

Guldberg P, Nedergaard T, Nielsen H J, Olsen A C, Ahrenkiel V, Zeuthen J

Abstract excerpt

We present a simple nonradioactive assay based on polymerase chain reaction (PCR) in combination with denaturing gradient gel electrophoresis (DGGE), which allows comprehensive mutation scanning of the entire coding sequence and splice junctions of the p53 gene in one analytical step. The key fea...

Topics

Aged
Aged, 80 and over
Algorithms
Base Sequence
Colorectal Neoplasms
DNA, Neoplasm
Electrophoresis, Polyacrylamide Gel
Female
Genes, p53
Humans
Male
Middle Aged

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Nucleic Acid Denaturation

Polymerase Chain Reaction

RNA Splicing

Rectal Neoplasms

Source

Open in PubMed

Identifiers

PMID: 9101296
DOI: 10.1002/(SICI)1098-1004(1997)9:4<348::AID-HUMU8>3.0.CO;2-1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9101296-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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