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Article

Rapid and efficient screening for p53 gene mutations by dideoxy fingerprinting.

BioTechniques - 1 Feb 1995

Blaszyk H, Hartmann A, Schroeder J J, McGovern R M, Sommer S S, Kovach J S

Abstract excerpt

Dideoxy fingerprinting (ddF) is an efficient method for detecting single base and other sequence changes in PCR-amplified DNA segments. This screening method is a hybrid between single-strand conformation polymorphism analysis (SSCP) and Sanger dideoxy sequencing. It involves a Sanger sequencing reaction with one dideoxynucleotide followed by non-denaturing gel electrophoresis. We are using ddF to screen for...

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PMID: 7727127

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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Rapid and efficient screening for p53 gene mutations by dideoxy fingerprinting.PMID 7727127Read ↗

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Relative efficiency of denaturing gradient gel electrophoresis and single strand conformation polymorphism in the detection of mutations in exons 5 to 8 of the p53 gene.Semantic proximity Read ↗

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