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Article

Rapid and efficient screening for p53 gene mutations by dideoxy fingerprinting.

BioTechniques - 1 Feb 1995

Blaszyk H, Hartmann A, Schroeder J J, McGovern R M, Sommer S S, Kovach J S

Abstract excerpt

Dideoxy fingerprinting (ddF) is an efficient method for detecting single base and other sequence changes in PCR-amplified DNA segments. This screening method is a hybrid between single-strand conformation polymorphism analysis (SSCP) and Sanger dideoxy sequencing. It involves a Sanger sequencing reaction with one dideoxynucleotide followed by non-denaturing gel electrophoresis. We are using ddF to screen for...

Topics

Breast Neoplasms
DNA Fingerprinting
Genes, p53
Genetic Carrier Screening
Genetic Testing
Mutation
Polymorphism, Genetic

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 7727127

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7727127-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Rapid and efficient screening for p53 gene mutations by dideoxy fingerprinting.PMID 7727127Read ↗

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