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Article

An "instant gene bank" method for heterologous gene cloning: complementation of two Aspergillus nidulans mutants with Gaeumannomyces graminis DNA.

Molecular & general genetics : MGG - 1 Feb 1994

Bowyer P, Osbourn A E, Daniels M J

Abstract excerpt

We present a novel technique for gene cloning by complementation of mutations in Aspergillus nidulans with DNA from a heterologous organism, Gaeumannomyces graminis. This technique bypasses the time-consuming and difficult construction of gene libraries, making it both rapid and simple. The method relies on recombination between a fungal replicating vector pHELP1 and linear G. graminis genomic DNA during...

Topics

Aspergillus nidulans
Base Sequence
Blotting, Southern
Cloning, Molecular
Fungi
Gene Library
Genes, Fungal
Genetic Complementation Test
Genetic Vectors
Molecular Sequence Data
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Plant Diseases

Plasmids

Restriction Mapping

Transformation, Genetic

Source

Identifiers

PMID: 8121400
DOI: 10.1007/BF00281795

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8121400-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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