Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Isolation by genetic complementation of two differentially expressed genes for beta-isopropylmalate dehydrogenase from Aspergillus niger.

Current genetics - 1 Sept 1996

Williams B A, Sillaots S, Tsang A, Storms R

Abstract excerpt

We have constructed an Aspergillus niger cDNA library with a yeast expression vector. The library DNA complemented a leucine auxotroph of Saccharomyces cerevisiae (strain BWG1-7a) at a frequency of 4x10(-4). Plasmids rescued from the yeast prototrophs also complemented Escherichia coli (strain MC1066) deficient in leucine biosynthesis. Sequence determination of the rescued plasmids revealed two genes for...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acid Sequence
Share to Base Sequence
Share to Codon

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Escherichia coli

Share to Genes, Fungal

Share to Genetic Complementation Test

Share to Molecular Sequence Data

Share to Mutation

Source

Identifiers

PMID: 8781173
DOI: 10.1007/s002940050137

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Isolation by genetic complementation of two differentially expressed genes for beta-isopropylmalate dehydrogenase from Aspergillus niger.PMID 8781173Read ↗

An "instant gene bank" method for heterologous gene cloning: complementation of two Aspergillus nidulans mutants with Gaeumannomyces graminis DNA.Semantic proximity Read ↗

An 'instant gene bank' method for gene cloning by mutant complementation.Semantic proximity Read ↗

Molecular analysis of a leu2-mutant of Candida maltosa demonstrates the presence of multiple alleles.Semantic proximity Read ↗

Direct Liquid-Culture Screening for Evaluating the Production of Heterologous Proteins Using an Auxotrophic Mutant of Aspergillus Oryzae.Semantic proximity Read ↗

Characterization of the Candida albicans TRP1 gene and construction of a homozygous trp1 mutant by sequential co-transformation.Semantic proximity Read ↗

Characterization of an efficient gene cloning strategy for Aspergillus niger based on an autonomously replicating plasmid: cloning of the nicB gene of A. niger.Semantic proximity Read ↗

Identification by functional analysis of the gene encoding alpha-isopropylmalate synthase II (LEU9) in Saccharomyces cerevisiae.Semantic proximity Read ↗

Cloning metabolic pathway genes by complementation in Escherichia coli. Isolation and expression of Plasmodium falciparum glucose phosphate isomerase.Semantic proximity Read ↗

Cloning heterologous genes: problems and approaches.Semantic proximity Read ↗

Characterization and overexpression of the Aspergillus niger gene encoding the cAMP-dependent protein kinase catalytic subunit.Semantic proximity Read ↗

Drug-induced phenotypes provide a tool for the functional analysis of yeast genes.Semantic proximity Read ↗

Correlation of biochemical blocks and genetic lesions in leucine auxotrophic strains of the imperfect yeast Candida maltosa.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.