Article
Isolation by genetic complementation of two differentially expressed genes for beta-isopropylmalate dehydrogenase from Aspergillus niger.
Current genetics - 1 Sept 1996
Williams B A, Sillaots S, Tsang A, Storms R
Abstract excerpt
We have constructed an Aspergillus niger cDNA library with a yeast expression vector. The library DNA complemented a leucine auxotroph of Saccharomyces cerevisiae (strain BWG1-7a) at a frequency of 4x10(-4). Plasmids rescued from the yeast prototrophs also complemented Escherichia coli (strain MC...
Topics
- 3-Isopropylmalate Dehydrogenase
- Alcohol Oxidoreductases
- Amino Acid Sequence
- Aspergillus niger
- Base Sequence
- Codon
- Escherichia coli
- Gene Dosage
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Genetic Complementation Test
