Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

An 'instant gene bank' method for gene cloning by mutant complementation.

Molecular & general genetics : MGG - 1 Feb 1994

Gems D, Aleksenko A, Belenky L, Robertson S, Ramsden M, Vinetski Y, Clutterbuck A J

Abstract excerpt

We describe a new method of gene cloning by complementation of mutant alleles which obviates the need for construction of a gene library in a plasmid vector in vitro and its amplification in Escherichia coli. The method involves simultaneous transformation of mutant strains of the fungus Aspergillus nidulans with (i) fragmented chromosomal DNA from a donor species and (ii) DNA of a plasmid without a selectable...

Topics

Aspergillus nidulans
Cloning, Molecular
DNA, Fungal
Escherichia coli
Gene Library
Genetic Complementation Test
Mutation
Penicillium
Plasmids
Restriction Mapping
Transformation, Genetic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 8121403
DOI: 10.1007/BF00281798

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8121403-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

An 'instant gene bank' method for gene cloning by mutant complementation.PMID 8121403Read ↗

An "instant gene bank" method for heterologous gene cloning: complementation of two Aspergillus nidulans mutants with Gaeumannomyces graminis DNA.Semantic proximity Read ↗

Characterization of an efficient gene cloning strategy for Aspergillus niger based on an autonomously replicating plasmid: cloning of the nicB gene of A. niger.Semantic proximity Read ↗

Rapid Gene Concatenation for Genetic Rescue of Multigene Mutants in Candida albicans.Semantic proximity Read ↗

Whole-genome combinatorial gene fusions generate novel genes for advanced microbial trait development.Semantic proximity Read ↗

A system for deletion and complementation of Candida glabrata genes amenable to high-throughput application.Semantic proximity Read ↗

Cloning yeast genes by complementation.Semantic proximity Read ↗

Isolation by genetic complementation of two differentially expressed genes for beta-isopropylmalate dehydrogenase from Aspergillus niger.Semantic proximity Read ↗

High-Copy Yeast Library Construction and High-Copy Rescue Genetic Screen in Saccharomyces cerevisiae.Semantic proximity Read ↗

Characterization of the Candida albicans TRP1 gene and construction of a homozygous trp1 mutant by sequential co-transformation.Semantic proximity Read ↗

Cercosporin-deficient mutants by plasmid tagging in the asexual fungus Cercospora nicotianae.Semantic proximity Read ↗

A versatile and efficient gene-targeting system for Aspergillus nidulans.Semantic proximity Read ↗

Cloning heterologous genes: problems and approaches.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.