Deletion of Aspergillus nidulans aroC using a novel blaster module that combines ET cloning and marker rescue.

doi:10.1007/s00438-002-0789-8

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Article

Deletion of Aspergillus nidulans aroC using a novel blaster module that combines ET cloning and marker rescue.

Molecular genetics and genomics : MGG - 1 Feb 2003

Krappmann S, Braus G H

Abstract excerpt

Blaster cassettes are of significant value in functional genomics, as they represent tools with which to inactivate duplicated or homologous genes in an individual organism. We have constructed a novel blaster module which allows repeated gene deletion in the filamentous fungus Aspergillus nidulans. Because bacterial resistance marker cassettes are employed as flanking repeats in direct orientation, the blaster...

Topics

Aspergillus nidulans
Base Sequence
Chorismate Mutase
Cloning, Molecular
DNA Primers
Gene Deletion
Gene Targeting
Genes, Fungal
Genetic Engineering
Genetic Markers
Genetic Techniques
Phenotype

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Identifiers

PMID: 12589442
DOI: 10.1007/s00438-002-0789-8

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