Article
Deletion of Aspergillus nidulans aroC using a novel blaster module that combines ET cloning and marker rescue.
Molecular genetics and genomics : MGG - 1 Feb 2003
Krappmann S, Braus G H
Abstract excerpt
Blaster cassettes are of significant value in functional genomics, as they represent tools with which to inactivate duplicated or homologous genes in an individual organism. We have constructed a novel blaster module which allows repeated gene deletion in the filamentous fungus Aspergillus nidulans. Because bacterial resistance marker cassettes are employed as flanking repeats in direct orientation, the blaster...
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