Article
New multi-marker strains and complementing genes for Aspergillus nidulans molecular biology.
Fungal genetics and biology : FG & B - 1 Feb 2018
Dohn James W, Grubbs Alexander W, Oakley C Elizabeth, Oakley Berl R
Abstract excerpt
Technical advances in Aspergillus nidulans enable relatively easy deletion of genomic sequences, insertion of sequences into the genome and alteration of genomic sequences. To extend the power of this system we wished to create strains with several selectable markers in a common genetic background to facilitate multiple, sequential transformations. We have developed an approach, using the recycling of the pyrG...
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