A reverse genetic approach for generating gene replacement mutants in Ustilago maydis.

doi:10.1007/s00438-004-1047-z

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Article

A reverse genetic approach for generating gene replacement mutants in Ustilago maydis.

Molecular genetics and genomics : MGG - 1 Sept 2004

Brachmann A, König J, Julius C, Feldbrügge M

Abstract excerpt

We describe a versatile strategy for generating gene replacement mutants in the phytopathogenic fungus Ustilago maydis. The system includes the choice of 32 different insertion cassettes for genetic engineering purposes, such as gene disruption and more sophisticated insertions of reporter genes, heterologous promoters or combinations of the two. PCR-amplified flanking sequences needed for homologous...

Topics

Base Sequence
Fungal Proteins
Gene Expression
Genes, Fungal
Genes, Reporter
Genetic Engineering
Genetic Techniques
Green Fluorescent Proteins
Mutagenesis, Insertional
Mutation
Pheromones
Plants
Plasmids
Polymerase Chain Reaction
Promoter Regions, Genetic
Recombinant Proteins
Recombination, Genetic
Ustilago

Source

Open in PubMed

Identifiers

PMID: 15316769
DOI: 10.1007/s00438-004-1047-z

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