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Establishing a versatile Golden Gate cloning system for genetic engineering in fungi.

Fungal genetics and biology : FG & B - 1 Jan 2014

Terfrüchte Marius, Joehnk Bastian, Fajardo-Somera Rosa, Braus Gerhard H, Riquelme Meritxell, Schipper Kerstin, Feldbrügge Michael

Abstract excerpt

The corn pathogen Ustilago maydis is a well-studied fungal model organism. Along with a broad set of experimental tools, versatile strategies for the generation of gene replacement mutants by homologous recombination in U. maydis have been developed. Nevertheless, the production of corresponding linear DNA constructs still constitutes a time-limiting step. To overcome this bottleneck, various resistance cassette...

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Identifiers

PMID: 24211735
DOI: 10.1016/j.fgb.2013.10.012

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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