Article
Requirement of the propeptide for in vivo formation of active yeast carboxypeptidase Y.
The Journal of biological chemistry - 4 Mar 1994
Ramos C, Winther J R, Kielland-Brandt M C
Abstract excerpt
Deletions have been constructed in the region encoding the 91-amino acid propeptide of the vacuolar enzyme carboxypeptidase Y of Saccharomyces cerevisiae, and in vivo effects of these mutations on the intracellular transport of the mutant proenzymes have been examined. Deletions did not include t...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Carboxypeptidases
- Cathepsin A
- DNA Restriction Enzymes
- Enzyme Precursors
- Genes, Fungal
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Oligodeoxyribonucleotides
- Restriction Mapping
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Sequence Deletion