Article
Biogenesis of the yeast vacuole (lysosome). The use of active-site mutants of proteinase yscA to determine the necessity of the enzyme for vacuolar proteinase maturation and proteinase yscB stability.
European journal of biochemistry - 1 Jul 1995
Rupp S, Wolf D H
Abstract excerpt
The activation process of vacuolar proteinases in the yeast Saccharomyces cerevisiae via precursor maturation is initiated by the PRA1/PEP4 gene product, proteinase yscA. Chromosomal deletion of the PRA1/PEP4 locus leads to accumulation of inactive pro-proteinases in the vacuole. Nine active-site...
Topics
- Alleles
- Aspartic Acid Endopeptidases
- Base Sequence
- Binding Sites
- Chromosome Mapping
- Enzyme Precursors
- Enzyme Stability
- Molecular Sequence Data
- Mutation
- Protein Processing, Post-Translational
- Saccharomyces cerevisiae