Article
Yeast carboxypeptidase Y vacuolar targeting signal is defined by four propeptide amino acids.
The Journal of cell biology - 1 Aug 1990
Valls L A, Winther J R, Stevens T H
Abstract excerpt
The amino-terminal propeptide of carboxypeptidase Y (CPY) is necessary and sufficient for targeting this glycoprotein to the vacuole of Saccharomyces cerevisiae. A 16 amino acid stretch of the propeptide was subjected to region-directed mutagenesis using randomized oligonucleotides. Mutations alt...
Topics
- Amino Acid Sequence
- Base Sequence
- Carboxypeptidases
- Cathepsin A
- Cloning, Molecular
- Enzyme Precursors
- Genes, Fungal
- Glycoproteins
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Phenotype
- Protein Processing, Post-Translational
- Restriction Mapping
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Vacuoles