Article
Yeast carboxypeptidase Y requires glycosylation for efficient intracellular transport, but not for vacuolar sorting, in vivo stability, or activity.
European journal of biochemistry - 8 May 1991
Winther J R, Stevens T H, Kielland-Brandt M C
Abstract excerpt
Functions of the carbohydrate side chains of the yeast vacuolar enzyme carboxypeptidase Y (CPY) were investigated by removal, through site-directed mutagenesis, of the sequences which act as signals for N-linked glycosylation. The mutant forms of the enzyme were analysed with respect to activity...
Topics
- Base Sequence
- Biological Transport
- Carboxypeptidases
- Enzyme Stability
- Glycosylation
- Molecular Sequence Data
- Mutation
- Tunicamycin
- Vacuoles
- Yeasts