Article
Use of CPY and its derivatives to study protein quality control in various cell compartments.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012
Stolz Alexandra, Wolf Dieter H
Abstract excerpt
Mutated derivatives of carboxypeptidase yscY (CPY) are potent substrates to study protein quality control and protein degradation in different cell compartments in yeast. Depending on the subcellular compartment of interest, the design of the model substrate used has to be adapted. Here, we describe the derivatives of CPY used in genetic screens based on a sensitive growth test in order to identify new components...
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