Defective transcriptional activation by diverse VP16 mutants associated with a common inability to form open promoter complexes.

Abstract excerpt

Three different types of VP16 mutants were assayed in vitro. These included deletion of the C-terminal activation subdomain and alterations in either the acidic or non-acidic components of the minimal activation domain. The mutant GAL4-VP16 proteins were found to be transcriptionally defective using a HeLa cell nuclear extract. In all three cases the loss of transcription activity was accompanied by a...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Fungal Proteins
• Share to HeLa Cells
• Share to Mutation