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Article

Structure of the VP16 transactivator target in the Mediator.

Nature structural & molecular biology - 1 Apr 2011

Milbradt Alexander G, Kulkarni Madhura, Yi Tingfang, Takeuchi Koh, Sun Zhen-Yu J, Luna Rafael E, Selenko Philipp, Näär Anders M, Wagner Gerhard

Abstract excerpt

The human Mediator coactivator complex interacts with many transcriptional activators and facilitates recruitment of RNA polymerase II to promote target gene transcription. The MED25 subunit is a critical target of the potent herpes simplex 1 viral transcriptional activator VP16. Here we determine the solution structure of the MED25 VP16-binding domain (VBD) and define its binding site for the N-terminal portion...

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Identifiers

PMID: 21378963
DOI: 10.1038/nsmb.1999
PMCID: PMC3076674

Genes

MED25

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

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Research trail→

Click a neighbor to travelStep 1 · 12 closest

Structure of the VP16 transactivator target in the Mediator.PMID 21378963Read ↗

Characterization of the interaction between the acidic activation domain of VP16 and the RNA polymerase II initiation factor TFIIB.Semantic proximity Read ↗

NMR structure of the complex between the Tfb1 subunit of TFIIH and the activation domain of VP16: structural similarities between VP16 and p53.Semantic proximity Read ↗

Amino acid substitutions in the herpes simplex virus transactivator VP16 uncouple direct protein-protein interaction and DNA binding from complex assembly and transactivation.Semantic proximity Read ↗

Direct interaction of RNA polymerase II and mediator required for transcription in vivo.Semantic proximity Read ↗

Respiratory Syncytial virus NS1 protein targets the transactivator binding domain of MED25Semantic proximity Read ↗

Host factors associated with either VP16 or VP16‐induced complex differentially affect HSV‐1 lytic infectionSemantic proximity Read ↗

Exploitation of the Mediator complex by virusesSemantic proximity Read ↗

Mediator-RNA Polymerase II Interactions Critical for Transcriptional Activation Are Mediated by the N-terminal Half of MED14 and C-terminal Domain of RPB1Semantic proximity Read ↗

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The Mediator Complex: Progress over the Past DecadeSemantic proximity Read ↗

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