Article
Transfection with a cDNA encoding a Ser31 or Ser34 mutant human dihydrofolate reductase into Chinese hamster ovary and mouse marrow progenitor cells confers methotrexate resistance.
Gene - 25 Feb 1994
Banerjee D, Schweitzer B I, Volkenandt M, Li M X, Waltham M, Mineishi S, Zhao S C, Bertino J R
Abstract excerpt
Chinese hamster ovary (CHO) DHFR- cells were converted into the DHFR+ phenotype when they were transfected with a mammalian expression vector carrying human dihydrofolate reductase-encoding cDNAs (DHFR) containing a Ser31 or a Ser34 mutation. Furthermore, transfection of these mutants into wild-type CHO cells resulted in resistance to high levels of methotrexate (MTX), indicating that these human variants can act...
Topics
- Animals
- Arginine
- Base Sequence
- Blotting, Northern
- Blotting, Southern
- Bone Marrow
- CHO Cells
- Colony-Forming Units Assay
- Cricetinae
- DNA, Complementary
- Drug Resistance