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Article

Construction of a dominant selectable marker using a novel dihydrofolate reductase.

Gene - 15 Mar 1992

Hussain A, Lewis D, Yu M, Melera P W

Abstract excerpt

Simian virus 40 promoter-enhancer-based mammalian expression plasmids using dihydrofolate reductase (DHFR)-encoding cDNA sequences originally isolated from two methotrexate (MTX)-resistant, DHFR-overproducing Chinese hamster lung cell lines were constructed. One, designated pSVA75, contains a DHFR cDNA that encodes leucine (Leu22) and corresponds to the wild type (wt), MTX-sensitive form of the enzyme [Melera et...

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Source

Identifiers

PMID: 1555767
DOI: 10.1016/0378-1119(92)90374-x

Genes

DHFR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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