Construction of a dominant selectable marker using a novel dihydrofolate reductase.

doi:10.1016/0378-1119(92)90374-x

Back to search

Article

Construction of a dominant selectable marker using a novel dihydrofolate reductase.

Gene - 15 Mar 1992

Hussain A, Lewis D, Yu M, Melera P W

Abstract excerpt

Simian virus 40 promoter-enhancer-based mammalian expression plasmids using dihydrofolate reductase (DHFR)-encoding cDNA sequences originally isolated from two methotrexate (MTX)-resistant, DHFR-overproducing Chinese hamster lung cell lines were constructed. One, designated pSVA75, contains a DHF...

Topics

Animals
Blotting, Southern
Cell Line
Cricetinae
Cricetulus
Genes, Dominant
Genetic Markers
Genetic Vectors
Mutation
Plasmids
Tetrahydrofolate Dehydrogenase
Transfection

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.