Article
Retroviral transduction of human CD34+ umbilical cord blood progenitor cells with a mutated dihydrofolate reductase cDNA.
Human gene therapy - 1 Jan 1998
Flasshove M, Banerjee D, Leonard J P, Mineishi S, Li M X, Bertino J R, Moore M A
Abstract excerpt
Umbilical cord blood cells (UCB) have become a major target population for experimental and clinical studies using transfer of genes involved in inborn enzymatic diseases. Cord blood contains hematopoietic progenitor cells at a high frequency, and expanding these cells ex vivo generates sufficien...
Topics
- Antigens, CD34
- DNA, Complementary
- Drug Resistance
- Fetal Blood
- Folic Acid Antagonists
- Gene Transfer Techniques
- Genetic Vectors
- Hematopoietic Stem Cells
- Humans
- Methotrexate
- Mutation
- Proviruses
- Retroviridae
- S Phase
- Tetrahydrofolate Dehydrogenase