Identification and characterization of a mutation in the dihydrofolate reductase gene from the methotrexate-resistant Chinese hamster ovary cell line Pro-3 MtxRIII.

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Article

Identification and characterization of a mutation in the dihydrofolate reductase gene from the methotrexate-resistant Chinese hamster ovary cell line Pro-3 MtxRIII.

The Journal of biological chemistry - 15 May 1990

Dicker A P, Volkenandt M, Schweitzer B I, Banerjee D, Bertino J R

Abstract excerpt

A methotrexate-resistant Chinese hamster ovary cell line (Pro-3 MtxRIII), resistant due to a low-level amplified, altered target enzyme, dihydrofolate reductase (DHFR), has been characterized on the molecular level. The cDNA and coding regions of all six DHFR exons were amplified in vitro using Taq polymerase and directly sequenced. Analysis of the Pro-3 MtxRIII DHFR cDNA demonstrated a C----T base transition at...

Topics

Amino Acid Sequence
Animals
Base Sequence
Binding Sites
Cell Line
Cricetinae
DNA
DNA-Directed DNA Polymerase
Drug Resistance
Female
Gene Amplification

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Kinetics

Leucine

Methotrexate

Molecular Sequence Data

Molecular Structure

Mutation

Ovary

Source

Open in PubMed

Identifiers

PMID: 2335526

Genes

DHFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2335526-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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