Transfection of DHFR- and DHFR+ mammalian cells using methotrexate-resistant mutants of mouse dihydrofolate reductase.

doi:10.1016/0014-5793(90)81213-8

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Article

Transfection of DHFR- and DHFR+ mammalian cells using methotrexate-resistant mutants of mouse dihydrofolate reductase.

FEBS letters - 3 Sept 1990

Thillet J, Pictet R

Abstract excerpt

Site-directed mutagenesis was used to generate mutants of mouse dihydrofolate reductase more resistant to methotrexate than the wild type enzyme. The mutant genes were used to transfect either DHFR- or DHFR+ cell lines. These mutants, as well as the wild type gene, were able to confer methotrexat...

Topics

Animals
Cell Line
Cricetinae
Cricetulus
Drug Resistance
Female
Kinetics
L Cells
Methotrexate
Mice
Mutation
Ovary
Plasmids
Recombinant Proteins
Tetrahydrofolate Dehydrogenase
Transfection

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