Article
Selection for the expression of one form of Chinese hamster dihydrofolate reductase over another during growth in methotrexate.
Gene - 8 Jul 1994
Hussain A, Yang H, Protzman J, Melera P W
Abstract excerpt
Two mammalian expression plasmids, each carrying a cDNA encoding a different allele of dihydrofolate reductase (DHFR) present in the Chinese hamster lung cell line DC-3F, were constructed. These simian virus 40 promoter-enhancer-based plasmids, designated pSVA75 and pSVMQ19, are identical except...
Topics
- Alleles
- Animals
- Asparagine
- Aspartic Acid
- Blotting, Southern
- CHO Cells
- Cell Division
- Cell Survival
- Cells, Cultured
- Cricetinae
- Cricetulus
- DNA, Complementary
- Methotrexate
- Plasmids
- Restriction Mapping
- Selection, Genetic
- Tetrahydrofolate Dehydrogenase
- Transfection