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Article

Selection for the expression of one form of Chinese hamster dihydrofolate reductase over another during growth in methotrexate.

Gene - 8 Jul 1994

Hussain A, Yang H, Protzman J, Melera P W

Abstract excerpt

Two mammalian expression plasmids, each carrying a cDNA encoding a different allele of dihydrofolate reductase (DHFR) present in the Chinese hamster lung cell line DC-3F, were constructed. These simian virus 40 promoter-enhancer-based plasmids, designated pSVA75 and pSVMQ19, are identical except for a G-->A transition at nucleotide 286 of the DHFR coding sequence. Due to this change, the enzyme expressed by...

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Source

Identifiers

PMID: 8039714
DOI: 10.1016/0378-1119(94)90390-5

Genes

DHFR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Selection for the expression of one form of Chinese hamster dihydrofolate reductase over another during growth in methotrexate.PMID 8039714Read ↗

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