Development of a retroviral construct containing a human mutated dihydrofolate reductase cDNA for hematopoietic stem cell transduction.

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Article

Development of a retroviral construct containing a human mutated dihydrofolate reductase cDNA for hematopoietic stem cell transduction.

Blood - 1 Jun 1994

Li M X, Banerjee D, Zhao S C, Schweitzer B I, Mineishi S, Gilboa E, Bertino J R

Abstract excerpt

A double-copy Moloney leukemia virus-based retroviral construct containing both the NeoR gene and a mutant human dihydrofolate reductase (DHFR) cDNA (Ser31 mutant) was used to transduce NIH 3T3 and mouse bone marrow (BM) progenitor cells. This resulted in increased resistance of these cells to me...

Topics

3T3 Cells
Animals
Bone Marrow Transplantation
DNA, Complementary
Hematopoietic Stem Cells
Humans
Methotrexate
Mice
Moloney murine leukemia virus
Mutation
Tetrahydrofolate Dehydrogenase
Transduction, Genetic

Source

Open in PubMed

Identifiers

PMID: 8193378

Genes

DHFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8193378-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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