Article
Targeted disruption within the CD3 zeta/eta/phi/Oct-1 locus in mouse.
The EMBO journal - 15 Feb 1994
Koyasu S, Hussey R E, Clayton L K, Lerner A, Pedersen R, Delany-Heiken P, Chau F, Reinherz E L
Abstract excerpt
To elucidate the role of the CD3 eta subunit of the T cell receptor (TCR) in thymic development, a CD3 eta -/- mouse was generated by gene targeting. Insertion of a neomycin resistance gene into exon 9 of the CD3 zeta/eta/phi locus disrupted expression of CD3 eta and CD3 phi without affecting the...
Topics
- Alleles
- Animals
- Base Sequence
- Blotting, Northern
- CD3 Complex
- DNA Primers
- DNA-Binding Proteins
- Female
- Flow Cytometry
- Host Cell Factor C1
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
- Molecular Sequence Data
- Mutation
- Octamer Transcription Factor-1
- RNA, Messenger