Article
Mutation of the alpha 2 domain disulfide bridge of the class I molecule HLA-A*0201. Effect on maturation and peptide presentation.
Human immunology - 1 Apr 1994
Warburton R J, Matsui M, Rowland-Jones S L, Gammon M C, Katzenstein G E, Wei T, Edidin M, Zweerink H J, McMichael A J, Frelinger J A
Abstract excerpt
A combination of saturation and site-directed mutagenesis was utilized to disrupt the alpha 2 domain disulfide bridge of HLA-A*0201. Mutation of cysteine 101 to a serine (C101S) or of cysteine 164 to alanine (C164A) decreased the rate of maturation of the heavy chain, the total amount of mature h...
Topics
- Amino Acid Sequence
- Antigen Presentation
- Blotting, Western
- Cell Line
- Cells, Cultured
- Cytotoxicity, Immunologic
- Disulfides
- Gene Products, pol
- HIV-1
- HLA-A Antigens
- Humans
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation