Article
A cluster of mutations in HLA-A2 alpha 2 helix abolishes peptide recognition by T cells.
Immunogenetics - 1 Jan 1991
Moots R J, Matsui M, Pazmany L, McMichael A J, Frelinger J A
Abstract excerpt
In order to investigate the regions of HLA-A2 that control peptide-specific cytotoxic T lymphocyte (CTL) recognition, 37 HLA-A2 genes coding for 50 point mutations that span the alpha 2 helix were synthesized by the technique of saturation mutagenesis. Twenty-nine of these genes, which code for 41 point mutations, were transfected into C1R cells and used as targets in cytotoxicity assays, in the presence of...
Topics
- Cell Line
- Gene Expression
- HLA-A2 Antigen
- Humans
- Influenza A virus
- Mutation
- Protein Conformation
- Structure-Activity Relationship
- T-Lymphocytes, Cytotoxic
- Transfection
- Viral Proteins