Article
Isolation of three classes of conditional lethal Chinese hamster ovary cell mutants with temperature-dependent defects in low density lipoprotein receptor stability and intracellular membrane transport.
The Journal of biological chemistry - 19 Aug 1994
Hobbie L, Fisher A S, Lee S, Flint A, Krieger M
Abstract excerpt
Fluorescence-activated cell sorting was used to isolate 19 independent, temperature-sensitive, low density lipoprotein (LDL) receptor-deficient Chinese hamster ovary cell mutants that define three recessive complementation groups, ldlE, ldlF, ldlG. LDL receptor activity, essentially normal at the...
Topics
- Animals
- Biological Transport
- CHO Cells
- Coated Pits, Cell-Membrane
- Cricetinae
- Cricetulus
- Genes, Lethal
- Genetic Complementation Test
- Humans
- Intracellular Membranes
- Membrane Glycoproteins
- Mutation
- Phosphatidylinositols
- Protein Processing, Post-Translational
- Receptors, LDL
- Temperature