Article
Isolation of NPC1-deficient Chinese hamster ovary cell mutants by gene trap mutagenesis.
Journal of biochemistry - 1 Jun 2001
Higaki K, Ninomiya H, Sugimoto Y, Suzuki T, Taniguchi M, Niwa H, Pentchev P G, Vanier M T, Ohno K
Abstract excerpt
Chinese hamster ovary cell mutants defective in the NPC1 gene (NPC1-trap) were generated by retrovirus-mediated gene trap mutagenesis from a parental cell line JP17 expressing an ecotropic retrovirus receptor. Insertion of the gene trap vector in the NPC1 gene and the absence of the gene product were verified by 5'RACE and immunological analyses, respectively. NPC1-trap cells showed intracellular accumulation of...
Topics
- Animals
- CCAAT-Enhancer-Binding Proteins
- CHO Cells
- Cholesterol
- Cricetinae
- DNA-Binding Proteins
- Down-Regulation
- Feedback
- Gene Expression Regulation
- Homeostasis
- Lipoproteins, LDL
- Mutagenesis, Insertional
- Mutation
- Niemann-Pick Diseases