Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Isolation of NPC1-deficient Chinese hamster ovary cell mutants by gene trap mutagenesis.

Journal of biochemistry - 1 Jun 2001

Higaki K, Ninomiya H, Sugimoto Y, Suzuki T, Taniguchi M, Niwa H, Pentchev P G, Vanier M T, Ohno K

Abstract excerpt

Chinese hamster ovary cell mutants defective in the NPC1 gene (NPC1-trap) were generated by retrovirus-mediated gene trap mutagenesis from a parental cell line JP17 expressing an ecotropic retrovirus receptor. Insertion of the gene trap vector in the NPC1 gene and the absence of the gene product were verified by 5'RACE and immunological analyses, respectively. NPC1-trap cells showed intracellular accumulation of...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CCAAT-Enhancer-Binding Proteins
Share to CHO Cells

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Feedback

Share to Lipoproteins, LDL

Share to Niemann-Pick Diseases

Share to Response Elements

Share to Retroviridae

Share to Sterol Regulatory Element Binding Protein 1

Source

Identifiers

PMID: 11388901
DOI: 10.1093/oxfordjournals.jbchem.a002932

Genes

NPC1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Isolation of NPC1-deficient Chinese hamster ovary cell mutants by gene trap mutagenesis.PMID 11388901Read ↗

Oxysterol-Binding Protein-Related Protein 1L Regulates Cholesterol Egress from the Endo-Lysosomal System.Semantic proximity Read ↗

Role of Niemann-Pick type C1 protein in intracellular trafficking of low density lipoprotein-derived cholesterol.Semantic proximity Read ↗

NPC1 and NPC2 regulate cellular cholesterol homeostasis through generation of low density lipoprotein cholesterol-derived oxysterols.Semantic proximity Read ↗

Amino acid substitution in NPC1 that abolishes cholesterol binding reproduces phenotype of complete NPC1 deficiency in mice.Semantic proximity Read ↗

Differential Trafficking Phenotypes of NPC1 Mutant Proteins Reveal Distinct Cholesterol Accumulation Profiles.Semantic proximity Read ↗

Niemann-Pick C1 disease gene: homology to mediators of cholesterol homeostasis.Semantic proximity Read ↗

New murine Niemann-Pick type C models bearing a pseudoexon-generating mutation recapitulate the main neurobehavioural and molecular features of the disease.Semantic proximity Read ↗

Trafficking defects in endogenously synthesized cholesterol in fibroblasts, macrophages, hepatocytes, and glial cells from Niemann-Pick type C1 mice.Semantic proximity Read ↗

Correction of apolipoprotein A-I-mediated lipid efflux and high density lipoprotein particle formation in human Niemann-Pick type C disease fibroblasts.Semantic proximity Read ↗

Reovirus infection is regulated by NPC1 and endosomal cholesterol homeostasisSemantic proximity Read ↗

Impaired ABCA1-dependent lipid efflux and hypoalphalipoproteinemia in human Niemann-Pick type C disease.Semantic proximity Read ↗

A second complementation class of cholesterol transport mutants with a variant Niemann-Pick type C phenotype.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.