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Article

Isolation by fluorescence-activated cell sorting of Chinese hamster ovary cell lines with pleiotropic, temperature-conditional defects in receptor recycling.

The Journal of biological chemistry - 25 Jun 1991

Cain C C, Wilson R B, Murphy R F

Abstract excerpt

We have isolated several Chinese hamster ovary cell lines with temperature-sensitive defects in the recycling of receptors after endocytosis. These cell lines were selected using fluorescence-activated cell sorting for retention of a pulse of labeled transferrin after a chase in the presence of unlabeled transferrin. One of these cell lines, TfT1.11, was selected for further characterization. In TfT1.11 the...

Topics

Adenosine Triphosphate
Animals
Cell Line
Cricetinae
Cricetulus
Endocytosis
Female
Flow Cytometry
Humans
Kinetics
Mutation
Ovary

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Receptors, Cell Surface

Temperature

Transferrin

alpha-Macroglobulins

Source

Identifiers

PMID: 1711039

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1711039-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Isolation by fluorescence-activated cell sorting of Chinese hamster ovary cell lines with pleiotropic, temperature-conditional defects in receptor recycling.PMID 1711039Read ↗

Isolation of a temperature-sensitive variant Chinese hamster ovary cell line with a morphologically altered endocytic recycling compartment.Semantic proximity Read ↗

The receptor-recycling and lysosome biogenesis mutant TfT1.11 belongs to a new complementation group, End6.Semantic proximity Read ↗

Isolation of three classes of conditional lethal Chinese hamster ovary cell mutants with temperature-dependent defects in low density lipoprotein receptor stability and intracellular membrane transport.Semantic proximity Read ↗

Impaired secretion and fluid-phase endocytosis in the End4 mutant of Chinese hamster ovary cells.Semantic proximity Read ↗

Over-expression of Rififylin, a new RING finger and FYVE-like domain-containing protein, inhibits recycling from the endocytic recycling compartment.Semantic proximity Read ↗

Novel method for isolating mammalian cells defective in fluid-phase endocytosis.Semantic proximity Read ↗

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Recovery of function in Chinese hamster ovary cell mutants with temperature-sensitive defects in vacuolar acidification.Semantic proximity Read ↗

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