Article
Novel method for isolating mammalian cells defective in fluid-phase endocytosis.
Somatic cell and molecular genetics - 1 Nov 1992
Wang R H, Colbaugh P A, Kuo P, Bau M Y, Poppe L M, Draper R K
Abstract excerpt
A new method for isolating mutants defective in fluid-phase endocytosis has been developed based on the observation that endocytosed horseradish peroxidase can be made lethal to cells. The method was used to isolate a mutant from Chinese hamster ovary cells, termed HRP-1, that was temperature-sen...
Topics
- 3,3'-Diaminobenzidine
- Animals
- CHO Cells
- Catalysis
- Cell Death
- Cell Line
- Cell Separation
- Cricetinae
- Endocytosis
- Genetic Complementation Test
- Golgi Apparatus
- Horseradish Peroxidase
- Hydrogen Peroxide
- Mutation
- Polymers
- Temperature