Article
Isolation of a temperature-sensitive variant Chinese hamster ovary cell line with a morphologically altered endocytic recycling compartment.
Journal of cellular physiology - 1 Jun 1993
McGraw T E, Dunn K W, Maxfield F R
Abstract excerpt
We have enriched a mutagenized population of Chinese hamster ovary (CHO) cells for those defective in endocytosis by selection for survival to treatment with transferrin (Tf)-ricin and Tf-diphtheria toxin conjugates. Surviving cells were screened with a fluorescently labeled Tf uptake assay to id...
Topics
- Animals
- Brefeldin A
- CHO Cells
- Cricetinae
- Cyclopentanes
- Cytoskeleton
- Diphtheria Toxin
- Endocytosis
- Ethyl Methanesulfonate
- Fluorescent Antibody Technique
- Golgi Apparatus
- Kinetics
- Lipoproteins, LDL
- Microtubules
- Mutagenesis
- Phenotype
- Ricin
- Temperature