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Article

Structures of replacement vectors for efficient gene targeting.

Journal of biochemistry - 1 Mar 1994

Horie K, Nishiguchi S, Maeda S, Shimada K

Abstract excerpt

The relationship between DNA structure of replacement vectors and gene targeting efficiency was studied using positive-negative selection. The vectors contained pBR322 DNA, a bacterial neomycin-resistance gene (neo) for positive selection, a herpes simplex virus (HSV) thymidine kinase gene (tk) for negative selection, and a mouse genomic fragment, including exons 1 to 3 of the transthyretin (ttr) gene. The neo...

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Identifiers

PMID: 8056760
DOI: 10.1093/oxfordjournals.jbchem.a124362

Genes

GANC

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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