Article
Structures of replacement vectors for efficient gene targeting.
Journal of biochemistry - 1 Mar 1994
Horie K, Nishiguchi S, Maeda S, Shimada K
Abstract excerpt
The relationship between DNA structure of replacement vectors and gene targeting efficiency was studied using positive-negative selection. The vectors contained pBR322 DNA, a bacterial neomycin-resistance gene (neo) for positive selection, a herpes simplex virus (HSV) thymidine kinase gene (tk) f...
Topics
- Animals
- Blotting, Southern
- Cloning, Molecular
- Drug Resistance
- Ganciclovir
- Gene Deletion
- Gene Transfer Techniques
- Genetic Engineering
- Genetic Vectors
- Mice
- Mutation
- Prealbumin
- Restriction Mapping
- Simplexvirus
- Thymidine Kinase
- Tumor Cells, Cultured