Article
High-efficiency oligonucleotide-directed plasmid mutagenesis.
Proceedings of the National Academy of Sciences of the United States of America - 1 Feb 1990
Olsen D B, Eckstein F
Abstract excerpt
A number of single- and double-base substitutions have been introduced into either the polylinker region or the lacZ gene in the plasmid vector pUC19. The efficiencies of these changes upon transfection of TG-1 bacterial cells were generally 70-80%. A strategy has been devised by which the wild-type DNA can be selectively destroyed. It is primarily based on the resistance of phosphorothioate internucleotide...
Topics
- Base Sequence
- DNA Restriction Enzymes
- Molecular Sequence Data
- Mutation
- Nucleic Acid Heteroduplexes
- Oligonucleotide Probes
- Plasmids
- Restriction Mapping