Article
Efficiency of chimeraplast gene targeting by direct nuclear injection using a GFP recovery assay.
Molecular therapy : the journal of the American Society of Gene Therapy - 1 Feb 2003
Tran Nam D, Liu Xiaoming, Yan Ziying, Abbote Duane, Jiang Qinshi, Kmiec Eric B, Sigmund Curt D, Engelhardt John F
Abstract excerpt
Traditional RNA-DNA chimeric oligonucleotides (chimeraplasts), composed of a continuous stretch of RNA and DNA residues in a duplex conformation, have been shown to correct single-base mutations in episomal and genomic DNA both in vitro and in vivo. In the current study, we have compared the efficiency of single-base pair correction between a traditionally designed chimeraplast (covalently linked duplex) and...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.