Article
Targeted introduction of a diphtheria toxin-resistant point mutation into the chromosomal EF-2 locus by in vivo homologous recombination.
Cell structure and function - 1 Dec 1991
Kido M, Miwatani H, Kohno K, Uchida T, Okada Y
Abstract excerpt
We have introduced a specific point mutation into the gene for chromosomal elongation factor 2 (EF-2) in Chinese hamster ovary cells (CHO-K1) by in vivo homologous recombination. To obtain a selectable construct for gene-targeting, we modified a diphtheria toxin-resistant mutant EF-2 gene (Gly717...
Topics
- Animals
- Blotting, Southern
- Cell Line
- Chromosome Mapping
- Cloning, Molecular
- DNA
- Diphtheria Toxin
- Drug Resistance
- Immunotoxins
- Mutation
- Neomycin
- Plasmids
- Recombination, Genetic
- Selection, Genetic
- Sequence Homology, Nucleic Acid