Article
Molecular characterization of thirteen gyrA mutations conferring nalidixic acid resistance in Bacillus subtilis.
Molecular & general genetics : MGG - 8 Jul 1994
Munakata N, Morohoshi F, Saitou M, Yamazaki N, Hayashi K
Abstract excerpt
We isolated 607 independent nalidixic acid-resistant mutants from Bacillus subtilis. A 163 bp DNA segment from a 5' portion of the gyrA gene was amplified from the DNA of each mutant strain. After heat denaturation, the product was subjected to gel electrophoresis to detect conformational polymor...
Topics
- Bacillus subtilis
- Base Sequence
- DNA Gyrase
- DNA Mutational Analysis
- DNA Primers
- DNA Topoisomerases, Type II
- Drug Resistance, Microbial
- Electrophoresis, Agar Gel
- Molecular Sequence Data
- Mutation
- Nalidixic Acid
- Phenotype
- Polymorphism, Genetic
- Transformation, Bacterial