Article
Quinolone resistance mutations in the DNA gyrase gyrA and gyrB genes of Staphylococcus aureus.
Antimicrobial agents and chemotherapy - 1 Sept 1994
Ito H, Yoshida H, Bogaki-Shonai M, Niga T, Hattori H, Nakamura S
Abstract excerpt
A 6.4-kb DNA fragment containing the DNA gyrase gyrA and gyrB genes was cloned and sequenced from the quinolone-susceptible Staphylococcus aureus type strain ATCC 12600. An expression plasmid was constructed by inserting the cloned genes into the Escherichia coli-S. aureus shuttle vector pAT19, a...
Topics
- Amino Acid Sequence
- Anti-Infective Agents
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- DNA Gyrase
- DNA Topoisomerases, Type II
- DNA, Bacterial
- Drug Resistance, Microbial
- Genes, Bacterial
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Oxolinic Acid
- Sequence Homology, Amino Acid
- Staphylococcus aureus
- Transformation, Bacterial