Article
Molecular cloning of the gyrA gene and characterization of its mutation in clinical isolates of quinolone-resistant Edwardsiella tarda.
Diseases of aquatic organisms - 28 Nov 2005
Shin Soon Bum, Yoo Min Ho, Jeong Joon Bum, Kim Young Min, Chung Joon-Ki, Huh Min Do, Komisar Jack L, Jeong Hyun Do
Abstract excerpt
Knowing the entire sequence of the gene encoding the DNA gyrase Subunit A (gyrA) of Edwardsiella tarda could be very useful for confirming the role of gyrA in quinolone resistance. Degenerate primers for the amplification of gyrA were designed from consensus nucleotide sequences of gyrA from 9 different Gram-negative bacteria, including Escherichia coli. With these primers, DNA segments of the predicted size were...
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