Article
Detection of gyrA and gyrB mutations in quinolone-resistant clinical isolates of Escherichia coli by single-strand conformational polymorphism analysis and determination of levels of resistance conferred by two different single gyrA mutations.
Antimicrobial agents and chemotherapy - 1 Aug 1995
Ouabdesselam S, Hooper D C, Tankovic J, Soussy C J
Abstract excerpt
Twelve quinolone-resistant clinical isolates of Escherichia coli (nalidixic acid MICs, 64 to 512 micrograms/ml; norfloxacin MICs, 0.25 to 8 micrograms/ml) were transformed with plasmid pJSW101 carrying the gyrA+ gene and with plasmid pJB11 carrying the gyrB+ gene to examine the proportion of gyrA...
Topics
- Amino Acid Sequence
- Anti-Infective Agents
- Base Sequence
- DNA, Bacterial
- Drug Resistance, Microbial
- Escherichia coli
- Fluoroquinolones
- Genes, Bacterial
- Genetic Complementation Test
- Microbial Sensitivity Tests
- Molecular Sequence Data
- Mutation
- Plasmids