Article
Detection of 4-quinolone resistance mutation in gyrA gene of Shigella dysenteriae type 1 by PCR.
Antimicrobial agents and chemotherapy - 1 Oct 1994
Rahman M, Mauff G, Levy J, Couturier M, Pulverer G, Glasdorff N, Butzler J P
Abstract excerpt
To study 4-quinolone resistance, the N-terminal coding region of gyrA from nalidixic acid-susceptible and -resistant isolates of Shigella dysenteriae type 1 was amplified by PCR, cloned, and sequenced. DNA sequence analysis of gyrA from nalidixic acid-resistant isolates revealed a C-to-T transition at nucleotide position 248 leading to a Ser-83-to-Leu substitution which was absent in susceptible clinical...
Topics
- 4-Quinolones
- Amino Acid Sequence
- Anti-Infective Agents
- Base Sequence
- DNA Gyrase
- DNA Topoisomerases, Type II
- Drug Resistance, Microbial
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction