Article
Co-expression of the herpes simplex virus thymidine kinase gene potentiates methotrexate resistance conferred by transfer of a mutated dihydrofolate reductase gene.
Gene therapy - 1 Jun 1997
Mineishi S, Nakahara S, Takebe N, Banerjee D, Zhao S C, Bertino J R
Abstract excerpt
We have previously shown that transfer of a mutated dihydrofolate reductase (DHFR) confers resistance to methotrexate (MTX) to infected cells. We report herein the construction of a retrovirus vector, DC/SV6S31tk, which carries the herpes simplex virus thymidine kinase gene (HSVtk) as well as the...
Topics
- 3T3 Cells
- Animals
- Antimetabolites, Antineoplastic
- Antiviral Agents
- Bone Marrow Cells
- Coculture Techniques
- Drug Resistance, Neoplasm
- Ganciclovir
- Gene Expression
- Gene Transfer Techniques
- Genetic Therapy
- Genetic Vectors
- Male
- Methotrexate
- Mice
- Mice, Inbred C57BL
- Mutation
- Retroviridae