Article
A comparison of the propensity for gene amplification between near-tetraploid and near-diploid V79 clones resistant to 150 nM methotrexate.
Carcinogenesis - 1 Mar 1996
Hashimoto M W, Nikaido O, Kobayashi N, Chang C C, Trosko J E, Mori T
Abstract excerpt
Among various 150-nM methotrexate-resistant (MTXr) V79 clones isolated, we found that two near-tetraploid clones as well as a near-diploid clone with amplification in the dihydrofolate reductase (dhfr) gene readily developed resistant to 40 000 nM MTX within 3 months during stepwise increased MTX...
Topics
- Animals
- Antimetabolites, Antineoplastic
- Cell Line
- Cricetinae
- Diploidy
- Drug Resistance
- Gene Amplification
- In Situ Hybridization, Fluorescence
- Methotrexate
- Microscopy, Confocal
- Phenotype
- Polyploidy
- Tetrahydrofolate Dehydrogenase