Mutations in the gene for human dihydrofolate reductase: an unlikely cause of clinical relapse in pediatric leukemia after therapy with methotrexate.

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Article

Mutations in the gene for human dihydrofolate reductase: an unlikely cause of clinical relapse in pediatric leukemia after therapy with methotrexate.

Leukemia - 1 Mar 1996

Spencer H T, Sorrentino B P, Pui C H, Chunduru S K, Sleep S E, Blakley R L

Abstract excerpt

Resistance to methotrexate (MTX) in some sublines of mammalian cells is reported to be due to one of the following amino acid substitutions in dihydrofolate reductase (DHFR) that lower inhibition by MTX: Gly15 to Trp, Leu22 to Arg or Phe or Phe31 to Trp or Ser. We have produced variants of human DHFR (hDHFR) with these substitutions by directed mutagenesis. Recombinant hDHFR variants expressed in Escherichia coli...

Topics

Adolescent
Animals
Antimetabolites, Antineoplastic
Base Sequence
Child
Child, Preschool
Drug Resistance, Neoplasm
Female
Genes
Genetic Vectors
Humans

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Infant

Male

Methotrexate

Mice

Mice, Inbred C57BL

Molecular Sequence Data

Mutation

Source

Open in PubMed

Identifiers

PMID: 8642859

Genes

DHFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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