Allelic variation in the dihydrofolate reductase gene at amino acid position 95 contributes to antifolate resistance in Chinese hamster cells.

Article

Allelic variation in the dihydrofolate reductase gene at amino acid position 95 contributes to antifolate resistance in Chinese hamster cells.

Cancer research - 15 Dec 1993

Yu M, Melera P W

Abstract excerpt

The Chinese hamster lung cell line DC-3F contains two polymorphic dihydrofolate reductase (DHFR) alleles that are defined by an Asp-Asn amino acid sequence difference at position 95 in protein. Previously, we reported that the antifolate-resistant subline DC-3F/A3 overexpressed a Leu22-->Phe muta...

Topics

Alleles
Animals
Biological Transport
CHO Cells
Cricetinae
DNA, Complementary
Drug Resistance
Folic Acid Antagonists
Methotrexate
Mutation
Structure-Activity Relationship
Tetrahydrofolate Dehydrogenase

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