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Article

Lack of somatic mutation in a kappa light chain transgene.

European journal of immunology - 1 Jun 1990

Sharpe M J, Neuberger M, Pannell R, Surani M A, Milstein C

Abstract excerpt

Analysis of mice transgenic for immunoglobulin genes should allow definition of the cis-acting DNA sequences required to target somatic mutation to antibody V genes. We have looked for mutations in a chimeric kappa transgene encoding a V region specific for the hapten 2-phenyloxazolone (phOx) linked to a rat C kappa gene. Two independent lines of transgenic mice were hyperimmunized with phOx and splenic...

Topics

Amino Acid Sequence
Animals
Base Sequence
Gene Rearrangement, B-Lymphocyte, Light Chain
Hybridomas
Immunization
Immunoglobulin Heavy Chains
Immunoglobulin kappa-Chains
Mice
Mice, Transgenic
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Oxazolone

Source

Identifiers

PMID: 2115000
DOI: 10.1002/eji.1830200625

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2115000-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Lack of somatic mutation in a kappa light chain transgene.PMID 2115000Read ↗

Somatic hypermutation of immunoglobulin kappa may depend on sequences 3' of C kappa and occurs on passenger transgenes.Semantic proximity Read ↗

A lambda 1 transgene under the control of a heavy chain promoter and enhancer does not undergo somatic hypermutation.Semantic proximity Read ↗

Analysis of hypermutation in immunoglobulin heavy chain passenger transgenes.Semantic proximity Read ↗

Analysis of somatic mutations in kappa transgenes.Semantic proximity Read ↗

Characterization of the cis-acting elements required for somatic hypermutation of murine antibody V genes using conventional transgenic and transgene homologous recombination approaches.Semantic proximity Read ↗

Somatic hypermutation of an immunoglobulin transgene in kappa transgenic mice.Semantic proximity Read ↗

Passenger transgenes reveal intrinsic specificity of the antibody hypermutation mechanism: clustering, polarity, and specific hot spots.Semantic proximity Read ↗

Allelic exclusion and control of endogenous immunoglobulin gene rearrangement in kappa transgenic mice.Semantic proximity Read ↗

Affinity maturation leads to differential expression of multiple copies of a kappa light-chain transgene.Semantic proximity Read ↗

Insertion of 2 kb of bacteriophage DNA between an immunoglobulin promoter and leader exon stops somatic hypermutation in a kappa transgene.Semantic proximity Read ↗

Somatic hypermutation of an immunoglobulin mu heavy chain transgene.Semantic proximity Read ↗

Hypermutation is observed only in antibody H chain V region transgenes that have recombined with endogenous immunoglobulin H DNA: implications for the location of cis-acting elements required for somatic mutation.Semantic proximity Read ↗

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