Affinity maturation leads to differential expression of multiple copies of a kappa light-chain transgene.

doi:10.1038/363271a0

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Article

Affinity maturation leads to differential expression of multiple copies of a kappa light-chain transgene.

Nature - 20 May 1993

Lozano F, Rada C, Jarvis J M, Milstein C

Abstract excerpt

Transgenic animals containing rearranged heavy or light chains are used to study the process of hypermutation, which characterizes the maturation of the antibody response. LK6 mice contain five copies of a transgene coding for a light chain produced in response to the hapten 2-phenyloxazolone. We...

Topics

Amino Acid Sequence
Animals
Antibody Affinity
Base Sequence
Cloning, Molecular
DNA
Down-Regulation
Gene Deletion
Gene Expression Regulation
Gene Rearrangement, B-Lymphocyte, Light Chain
Haptens
Hybridomas

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Immunoglobulin kappa-Chains

Mice

Mice, Transgenic

Molecular Sequence Data

Mutation

Oxazolone

Source

Open in PubMed

Identifiers

PMID: 8487865
DOI: 10.1038/363271a0

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8487865-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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Affinity maturation leads to differential expression of multiple copies of a kappa light-chain transgene.PMID 8487865Read ↗

Analysis of hypermutation in immunoglobulin heavy chain passenger transgenes.Semantic proximity Read ↗

Passenger transgenes reveal intrinsic specificity of the antibody hypermutation mechanism: clustering, polarity, and specific hot spots.Semantic proximity Read ↗

Somatic hypermutation.Semantic proximity Read ↗

Somatic hypermutation of immunoglobulin kappa may depend on sequences 3' of C kappa and occurs on passenger transgenes.Semantic proximity Read ↗

Lack of somatic mutation in a kappa light chain transgene.Semantic proximity Read ↗

A lambda 1 transgene under the control of a heavy chain promoter and enhancer does not undergo somatic hypermutation.Semantic proximity Read ↗

Somatic hypermutation in mouse lambda chains.Semantic proximity Read ↗

Decreased frequency of somatic hypermutation and impaired affinity maturation but intact germinal center formation in mice expressing antisense RNA to DNA polymerase zeta.Semantic proximity Read ↗

Low antigen dose favours selection of somatic mutants with hallmarks of antibody affinity maturation.Semantic proximity Read ↗

Somatic hypermutation of an immunoglobulin mu heavy chain transgene.Semantic proximity Read ↗

Visualizing the effects of antigen affinity on T‐dependent B‐cell differentiationSemantic proximity Read ↗

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