Article
Passenger transgenes reveal intrinsic specificity of the antibody hypermutation mechanism: clustering, polarity, and specific hot spots.
Proceedings of the National Academy of Sciences of the United States of America - 15 Mar 1993
Betz A G, Rada C, Pannell R, Milstein C, Neuberger M S
Abstract excerpt
We have analyzed somatic hypermutation in mice carrying an immunoglobulin kappa transgene in order to discriminate mutations that reflect the intrinsic specificity of the hypermutation mechanism from those highlighted by antigenic selection. We have immunized animals with three different immunoge...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Bordetella pertussis
- DNA
- Enzyme-Linked Immunosorbent Assay
- Genes, Immunoglobulin
- Immunoglobulin Variable Region
- Immunoglobulin kappa-Chains
- Mice
- Mice, Transgenic
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides